What is gel chromatography?
gel chromatography, also called Gel Filtration, in analytical chemistry, technique for separating chemical substances by exploiting the differences in the rates at which they pass through a bed of a porous, semisolid substance.
What are applications of gel chromatography?
Applications of Gel Filtration Chromatography It is used in protein fractionation experiments. Gel filtration technique is also used in molecular weight determination. Separation of sugar, proteins, peptides, rubbers, and others on the basis of their size.
Why buffer is used in gel chromatography?
For gel filtration chromatography, Tris buffer or sodium phosphate buffer is most commonly used. An ionic strength of at least 0.05 M is recommended to reduce nonspecific interactions between the proteins being separated and the chromatographic matrix.
Why buffer is used in gel filtration chromatography?
Which detector is used in gel-filtration?
The detectors may be concentration-sensitive detectors, bulk property detectors, refractive index (RI) detectors, etc.
What is column volume in HPLC?
The void volume of a column is also referred to as the dead volume, the dwell volume, or simply the column volume. Whichever way it’s referred to, this volume means the volume of the column hardware minus the volume occupied by the particles packed inside.
What is BV unit?
Biological value, a measure of the proportion of absorbed protein from a food which becomes incorporated into the proteins of the organism’s body. Binocular vision, vision in which both eyes are used together.
How does gel filtration chromatography work?
There are two extremes in the separation profile of a gel filtration column.
What are the uses of chromatography?
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What is gel permeation chromatography?
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Is HPLC chromatography reliable?
They are both reliable, depend on your analyte of interest if volatile GC is suitable, While HPLC most suitable for non volatile and semi volatile